Society of Feeding Mind, Combating Malnutrition
Egyptian Journal of Nutrition and Health
1687-7950
2535-1559
9
1
2014
01
01
Effect of diets fortified with Nigella sativa, sesame seeds and high calcium on osteoporosis in ovariectomized rats
1
17
EN
Reham
Ramadan
Nutrition and Food Sciences Department, Faculty of Home Economics, Helwan University
10.21608/ejnh.2014.4813
The study was carried out to assess the effect of diets fortified with Nigella sativa (NS), sesame (SS) seeds and high calcium (Ca) on osteoporosis in ovariectomized (OVX) rats. Fifty six rats were randomized into 8 equal groups (n= 7) and housed individually in metal metabolic cages. Under ether anesthesia, group (1) was SHAM-operated and fed on basal diet, while the other 7 groups were bilaterally OVX-operated. Three weeks after ovariectomy, group (2) was fed on basal diet, while groups (3), (4), (5), (6) and (7) were fed on experimental diets fortified with the following: 10% NS; 10% SS; 2% Ca; 10% NS+2% Ca and 10% SS+2°/0 Ca, respectively for 6 weeks. Group (8) was fed on basal diet and given orally Alendronate (anti-osteoporotic drug) in a daily dose of 3 mg/kg for 6 weeks. During feeding period, initial and final body weights of rats were recorded. At the end of the experiment, the last 24 hr urine samples were collected for estimating Ca, P, pyridinoline (PD) and deoxypyridinoline (DPD) concentrations. Rats were then euthanized by prolonged exposure to anesthetic ether. Blood samples were collected for estimating serum calcium (Ca), phosphorous (P), bone-specific alkaline phosphatase (b-ALP) and osteocalcin (OC) levels. The uteri were dissected out and weighed. Femur bones also were dissected out and used for measurement and analysis of bone ash. The results showed that diets fortified with NS and SS and high calcium prevented the increase in body weight gain and the decrease in uterine weight induced by ovariectomy. These experimental diets normalized serum Ca, P, b-ALP and OC levels and decreased urinary excretion of Ca, P, PD and DPD. There were also significant increases in femur bone mineral density (BMD) and calcium content in bone ash. In conclusion, diets fortified with NS, SS and high calcium have a promising anti-osteoporotic effect in OVX rats. The study recommends enrichment of the bakeries and food stuff with Nigella sativa and sesame seeds and eating meals rich in calcium such as dairy products (milk and yoghurt) may be beneficial for the treatment of postmenopausal osteoporosis in women.
Nigella Sativa,Sesame seeds,High-calcium diet,Ovariectomy,Osteoporosis,Biochemical analysis,Bone mineralization
https://ejnh.journals.ekb.eg/article_4813.html
https://ejnh.journals.ekb.eg/article_4813_abe9b452aad781fb97f4f1eabe670393.pdf
Society of Feeding Mind, Combating Malnutrition
Egyptian Journal of Nutrition and Health
1687-7950
2535-1559
9
1
2014
01
01
Efficacy of Ganoderma ( Ganoderma lucidum ) Against Nephrotoxicity Induced by Cisplatin in Male Rats
1
14
EN
Maha
Essam El-Din
Department of Nutrition and Food Science, Faculty of Home Economics, Helwan University, Cairo , Egypt.
Maha afy
El-Mowafy
Dietitian , Directorate of Education , Gharbiyah , Egypt
10.21608/ejnh.2014.4814
In traditional Chinese medicine, ganoderma lucidum are widely used as medicine for a variety of diseases, such as chronic bronchitis inflammation, hyperlipidemia, hypertension, neurasthenia, hepatitis, leukopenia, and adjuvant treatment of cancer . The aim of this study was to evaluate its effect against cisplatin - induced nephrotoxicity in adult male albino rats . Forty five animals were classified into 5 groups 9 rats for each group . Group (1) fed on basal diet (negative group); groups (2 - 5) were injected with cisplatin ( 5 mg / kg body wt. ) intraperitoneuly to induce nephrotoxicity in rats ; group ( 2 ) fed on the basal diet as positive control group (+Ve) ; remained groups fed on the basal diet and supplemented with ganoderma lucidum at 5 , 10 and 15 g / kg diet , respectively . Results revealed that positive control group had significant increase in serum levels of creatinine , urea , uric acid , urea nitrogen and total protein as well as Malondialdahyde ( MOD ) but had significant decrease in serum levels of Superoxide Dismutase (SOD) , Catalase (CAT) , Glutathione Peroxidase (GPX) and Glutathione (GSH) as compared to the negative control groups. While supplemented with different levels of ganoderma lucidum caused significant decreased in serum levels of creatinine , urea , uric acid , urea nitrogen and total protein as well as ( MOD ) , but had significant increased in serum levels of (SOD) , (CAT) , (GPX) and (GSH) as compared to the positive control group. From the present results it can be concluded that ganoderma lucidum feeding possess a therapeutic effect against cisplatin that induced nephrotoxicity in rats and inhibit oxidative stress. According to above results the consumption of ganoderma lucidum can be used to treat nephrotoxicity
male Albino rats,Cisplatin,Nephrotoxicity,Reishi mushrooms,Ganoderma lucidum,Kidney function,antioxidant markers
https://ejnh.journals.ekb.eg/article_4814.html
https://ejnh.journals.ekb.eg/article_4814_9307b68f866f0fc12096ab318609bc4a.pdf
Society of Feeding Mind, Combating Malnutrition
Egyptian Journal of Nutrition and Health
1687-7950
2535-1559
9
1
2014
01
01
Effect of low fat high protein diets in the presence of Arabic gum on weight loss of obese rats.
1
19
EN
Mona
Abd El-Basset
Home Economic Dept., Faculty of Specifie Education, Fayoum University.
10.21608/ejnh.2014.4815
Obesity is considered a serious disease affecting a large population worldwide. The present work was conducted to study the effect of low fat high protein diets in the absence or presence of Arabic gum on weight loss, some nutritional and biochemical parameters of obese rats. Normal male's albino rats (49) of Sprague Dawley Strain weighted 130 ± 5 g. were used in this study. The rats were divided into two main groups. The first main group (7 rats) fed on basal diet as a control negative group. The second main group (42 rats) were fed on high fat diet (HFD) for four weeks to induce obesity. After this period, the mean values of serum cholesterol, triglycerides and body weight gain were determined in the first and second main group to ensure obesity induction. The rats in the second main group was divided into seven subgroups (n=7rats) as follow: Subgroup (1) were fed on HFD as a control positive group, subgroup (2) were fed on HFD containing 5% Arabic gum AG, subgroups (3 and 4) were fed on low and very low caloric diets respectively [through the replacement of some dietary fat (saturated fat) with protein], subgroups (5 and 6) were fed on low fat high protein diets respectively and containing the same amount of AG used in subgroup (2),. The results in this study revealed that, the mean value of feed intake, body weight gain relative, liver weight decreased in all treated groups, as compared to obese groups. The mean values of serum glucose, leptin hormone, all lipid fractions (cholesterol, triglycerides, LDL-c, the ratio between LDL-c/HDL-c and VLDL-c), kidney functions (uric acid, urea nitrogen and creatinine) and liver enzymes (aspartate amino transferase AST, alanine amino transferase ALT and alkaline phosphatase ALP) decreased significantly p<0.05 in all tested groups, while HDL-c increased in all treated groups, as compared to the obese group (control +ve group). The best results recorded for the groups treated with low and very low fat high protein diets containing AG. It is concluded supplemented rat high protein low fat diet with Arabic gum improved<br />the nutritional and biological parameters of obese rats.
Rats,Obesity,high protein diets,high fat diets,Arabic gum,glucose,leptin hormone,Lipid profile,kidney functions,liver enzymes
https://ejnh.journals.ekb.eg/article_4815.html
https://ejnh.journals.ekb.eg/article_4815_fd273326cf75b5afb949e03f358be3ae.pdf
Society of Feeding Mind, Combating Malnutrition
Egyptian Journal of Nutrition and Health
1687-7950
2535-1559
9
1
2014
01
01
Inhibitory Effects of Grape Seeds Powder, Extract and Oil on Gentamicin Induced Nephrotoxicity in Rats
1
20
EN
Nagib
M
Home Economics Dept., Faculty of Specific Education, Mansoura University, Mansoura, Egypt
10.21608/ejnh.2014.4816
This study was designed to determine the Inhibitory effect of grape seeds powder, extract and oil on gentamicin-induced nephrotoxicity in rats.The study was carried out on 30 adult albino male rats Sprague-Dawley strain were classified into two groups: The first group (n= 6) left as normal control group(-ve), fed on basal diet, the second group(n= 24) injected with gentamicin (100 mg/kg/day for 7 days i.p). These animals were divided into 4 groups 6 rats each of these , control (+ve) group and 3 groups were treated with grape seeds powder ( 10 % of diet ) , grape seeds extract (300mg/kg b.w.orally by stomach tube) and grape seeds oil (4 ml/kg b.w. orally by stomach tube) respectively. The treatment period was designed for eight weeks. The results revealed that, Gentamicin injection induced marked nephrotoxicity as evidenced by significant elevation in serum levels of creatinine, urea, uric acid, A/G ratio, MDA ,renin hormone, phosphorus and potassium, with significant reduction in serum levels of total protein, albumin, globulin, (SOD), (GPX), (GST) ,catalase antioxidant enzymes, erythropoietin hormone (EPO) , vitamin D and Ca . While the rat groups treated with grape seeds powder ,extract and oil showed significant improvement of the levels of serum total protein, albumin, globulin, kidney tissue superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione transferase (GST) , catalase antioxidant enzymes, erythropoietin hormone (EPO) and vitamin D, and significant decrease in serum creatinine, urea, uric acid, albumin/globulin ratio ,kidney tissue malondialdehyde (MDA), renin hormone, phosphorus and potassium compared with control(+ve) group. In conclusion, feeding rats with grape seeds powder ,extract and oil may significantly reduce nephrotoxicity, So this study recommend the use of grape seeds especially grape seeds oil or grape seeds extract for humans suffering nephrotoxicity, could be of value.
grape seeds,Grape seeds extract,oil,Nephrotoxicity,kidney damage,gentamicin,Rats
https://ejnh.journals.ekb.eg/article_4816.html
https://ejnh.journals.ekb.eg/article_4816_354558df6b27b02067d1fbd8d69ce1b3.pdf
Society of Feeding Mind, Combating Malnutrition
Egyptian Journal of Nutrition and Health
1687-7950
2535-1559
9
1
2014
01
01
Chemical constituents of Eruca sativa and treatment activity against paracetamol inducing hepatic injury in experimental rats
1
12
EN
M
ELSadek
Nutrition and Food Science Department, Faculty of Home Economics, Helwan University .
10.21608/ejnh.2014.4817
In the present study, gross chemical composition and some minerals content of Eruca saliva leaves and seeds were estimated. Also, hepatotreatment activity of Eruca sativa leaves, juice, oil and seeds against paracetamol induced liver damage in rats were investigated. Results of this study revealed that Eruca saliva (rocket salad) seeds had higher content of protein, fat and carbohydrate and minerals (Zn, Fe,Ca, K and Cu). Eruca sativa leaves had higher content of fibre, ash and moisture. Paracetamol induced liver injury rat groups treated with Eruca sativa leaves, juice, oil and seeds improved weight gain percent and food intake that appeared within normal. However, they decreased total serum protein and urea with significant increase in total bilirubin, haemoglobin (Hb) and packed cell volume (PCV) compared with control (+ve) group. Moreover, they lowered liver function enzymes, liver cholesterol, total lipids and MDA and elevated liver triglyceride, glycogen and liver antioxidant enzymes compared with control (+ve) group.<br />In conclusion, Eruca sativa could could help the liver against paracetamol induced hepatic injury through its potent antioxidant activity in rats.
Eruca sativa,Liver Injury,Paracetamol,liver enzymes,antioxidant,Rats
https://ejnh.journals.ekb.eg/article_4817.html
https://ejnh.journals.ekb.eg/article_4817_e0fc3a5c8e9be856adbae2f865aabe50.pdf
Society of Feeding Mind, Combating Malnutrition
Egyptian Journal of Nutrition and Health
1687-7950
2535-1559
9
1
2014
01
01
Control of Hyperlipidemia in Rats By Using Papaya and Stirred Yoghurt
1
24
EN
Nagib
M
Home Economics Dept., Faculty of Specific Education, Mansoura University, Mansoura, Egypt
El- Kewawy
E
Home Economics Dept., Faculty of Specific Education, Mansoura University, Mansoura, Egypt
10.21608/ejnh.2014.4818
Current research aims to study the effect of feeding on stirred yoghurt only, papaya only and stirred yoghurt supplemented with 20% papaya on hyperlipidemia male rats. The study was conducted on 36 male albino rats divided into two main groups, the first main group 6 rats were fed on basal diet only and contrived as control group, while the second main group 30 rats with induced hypercholesterolemia through nutrition on a diet high in cholesterol content1%, the second group were divided into five equal subgroups and fed for 2 months.one subgroup was left as a positive control group (Group 1),whereas group (2)was treated with atorvastatin (10 mg/kg) orally, while groups(3,4,5) have been fed adding stirred yoghurt only , papaya only and stirred yoghurt supplemented with 20% papaya by rate (5m1/kg b.w) orally using a stomach tube daily for 8 weeks. The results revealed that all treated rat groups(experimental groups) showed a significant decrease in TC , TG , LDL-C ,VLDL-C homocysteine, TC / HDL-C and LDL-C / HDL-C than positive control group , while HDL-c was increased , in compared with the control (+ve) group. As a result, the best values of these variables appeared in stirred yoghurt supplemented with 20% papaya , atorvastatin and papaya only in compared with the other treated groups .The results revealed that liver enzymes (AST, ALT) showed significant decrease in all treated rat groups , All treated groups showed a significant increase in SOD, GSH and CAT and showed a significant decrease in MDA compared with the control (+ve) group. Histopathological examination of the rat's hearts confirmed this amelioration in rats group that consumed stirred yoghurt supplemented with 20% papaya .<br />The study recommended the use of stirred yoghurt supplemented with 20% papaya in patients meals suffering from hyperlipidemia.
Stirred Yoghurt,Papaya,hyperlipidemia,Cholesterol,triglyceride,liver functions- antioxidant enzymes
https://ejnh.journals.ekb.eg/article_4818.html
https://ejnh.journals.ekb.eg/article_4818_68aa037195c760fc75eb6873c78dedbc.pdf